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Title   골 거대세포종에서 재발 예측인자로서의 병리조직학적인 소견, DNA 배수성, Proliferating Cell Nuclear Antigen 양성율, C-fos 암유전자 및 형질전환성장인자 -a와 -B의 발현 ( Prognostic Significance of Histologic Features, DNA Content, Expression of Proliferatin Cell Nuclear Antigen (PCNA), c-fos Protein and Tra
Publicationinfo   1997 Jan; 029(02): 266-280.
Key_word   Bone, Giant cell tumor, PCNA, TGF-a & B, Ploidy pattern
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Abstract   Purpose: This study was attempted to investigate the prevalence of the expression of c-fos protein, TGF-a and -B, PCNA, DNA ploidy pattern and histopathological parameters of giant cell tumor (GCT) of bone and to correlate with prognosis and to extend our understanding on tumorigenesis of GCT. Materials and Methods: Twenty eight cases of paraffin-embedded tissue were studied, classified as recurrent (5 cases) and non-recurrent group (12cases) within the limits of the cases which afforded surgical material on first operation. Results: No significant difference was observed in cellularity of stromal cells, atypia of stromal and giant cells, presence of hemorrhage and necrosis between recurrent and non-recurrent group. However, presence of more than 10 mitotic figures in 10 high power fields in recurrent group was significantly higher than non-recurrent group (P<0.05). The immunoreactivity for PCNA was seen only in nuclei of stromal cells, whereas nuclei of giant cells showed negative staining. The positivity of PCNA revealed no significant difference between non-recurrent (mean; 40.9%) and recurrent group (34.4%). The expression of c-fos oncogene was seen in 5 cases (100%) in recurrent group, and 8 cases (66.7%) in non-recurrent group, and no significant difference was seen. No significant difference of expression of TGF-a was seen in 5 cases (100%) in recurrent group and in 11 cases (91.7%) in non-recurrent group. The expression of TGF-B in stromal cells was significantly higher in non-recurrent group (80%) compared to recurrent group (100%) (P<0.05). In DNA analysis out of 18 cases, 4 cases (22.2%) were aneuploidy and 14 cases (77.8%) were diploidy. Among 4 aneuploidy cases, 3 cases (75%) had no recurrence, and 1 case (25%) had metastasis to lung and expired. No significant difference of DNA ploidy pattern was seen between the recurrent and non-recurrent group. Conclusion: Presence of more than 10 mitotic figures in 10 high power fields and less expression of TGF-B are related to higher possibility of recurrence and it is suggested that the number of mitotic figure (more than 10/10HPF) and expression of TGF-B could be helpful parameters in predicting recurrence of GCT.
저 자   장희경(Hee Kyung Jang),윤성훈(Sung Hoon Yoon),김재도(Jae Do Kim),허만하(Man Ha Heo)